Biological kit for separating electronegative low density lipoprotein from specimen, reagent solution and method for separating electronegative low density lipoprotein from specimen

A method for separating an electronegative low density lipoprotein from a specimen, comprising:

• providing the specimen which comprises a mixture of lipoproteins;
• adding a Reagent B, a Reagent C and a Reagent D to the specimen before subjecting the specimen to a first centrifugation at a first speed for at least 6 hours to obtain a sample, wherein the Reagent B has a density from 1.090 g/cm3 to 1.093 g/cm3 and comprises a first buffer, a second buffer and a salt, the Reagent C has a density from 1.060 g/cm3 to 1.063 g/cm3 and comprises the first buffer, the second buffer, glycerol and the salt, and the Reagent D has a density from 0.98 g/cm3 to 1.001 g/cm3 and comprises the first buffer and the second buffer;
• removing a first portion of the sample and with a density from 0.960 g/cm3 to 1.006 g/cm3 from the sample;
• removing a second portion of the sample and with a density from 1.006 g/cm3 to 1.019 g/cm3 from the sample;
• removing a third portion of the sample and with a density from 1.019 g/cm3 to 1.031 g/cm3 from the sample;
• removing a fourth portion of the sample and with a density from 1.031 g/cm3 to 1.036 g/cm3 from the sample;
• removing a fifth portion of the sample and with a density from 1.036 g/cm3 to 1.063 g/cm3 from the sample;
• and
• subjecting at least one of the first portion, the second portion, the third portion, the fourth portion and the fifth portion to a dialysis to obtained at least one purified lipoprotein mixture corresponding to at least one of the first portion, the second portion, the third portion, the fourth portion and the fifth portion, wherein at least one of the third portion, the fourth portion and the fifth portion comprises an electronegative low density lipoprotein.