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US 2025/0027168 A1Published application
Detecting disease-associated target nucleic acids in a mammal and treatment thereof
OwnerRobert Cooper, Jeff Hasty +3inventors
InventorsRobert Cooper, Jeff Hasty, Daniel Lindsay Worthley, Susan Lesley Woods, Josephine Wright
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Abstract
Disclosed herein are methods of detecting a target nucleic acid in a sample (e.g., biological sample or environmental sample). The method includes administering to the sample an effective amount of a composition comprising an engineered bacterium (e.g., A. baylyi) and detecting an output signal. In some cases, the mammal has a cancer or an infectious disease.
Claims26
A method of detecting a target nucleic acid in a sample, the method comprising:
- a) administering to the sample an effective amount of a composition comprising a bacterium, wherein the bacterium comprises:
- a competence system comprising a homologous recombination construct, wherein a first homology arm is substantially complementary to a sequence upstream of the target nucleic acid and a second homology arm is substantially complementary to a sequence downstream of the target nucleic acid,
- an output gene construct encoding an output signal, and optionally,
- a CRISPR/Cas system comprising an effector nuclease and a spacer directed to a non-targeted nucleic acid, and
- b) detecting the output signal.
2 more elements
Dependencies are inferred from the claim text.