Method for selecting target sites for site-specific genome modification in plants
AssigneeMonsanto Technology LLC
InventorsBrent BROWER-TOLAND, Paul S. CHOMET, Robert T. GAETA, Andrei Y. KOURANOV, Jonathan C. LAMB, Richard J. LAWRENCE, Ruth WAGNER
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Abstract
The present disclosure provides methods and compositions for identification of optimal genomic loci in plant genome for site-directed integration in plants.
Claims29
A recombinant sequence comprising a non-genic plant genomic sequence and a DNA of interest, wherein the DNA of interest is integrated into a target site in the non-genic plant genomic sequence, and wherein the target site is located in a haplotype window associated with a neutral to positive impact on one or more agronomic traits, wherein the target site is further located at genetic distance greater than 1 cM of a haplotype window that is associated with a negative impact on one or more agronomic traits and wherein the non-genic plant genomic sequence is a corn genomic sequence selected from the group consisting of SEQ ID NOs:
- 123-172, 294, 299-551, 555 and 556 or a soybean genomic sequence selected from the group consisting of SEQ ID NOs:252-282 and 554.
-8. (canceled).
A method of making a transgenic plant cell comprising a DNA of interest targeted to at least one non-genic plant genomic sequence, the method comprising:
- i. selecting a target site located within a haplotype window associated with a neutral to positive impact on one or more agronomic traits;
- ii. introducing a site-specific genome modification enzyme into a plant cell, wherein the site-specific genome modification enzyme cleaves the target site in the non-genic plant genomic sequence;
- iii. introducing a DNA of interest;
- iv. targeting the DNA of interest to the target site, wherein the cleavage of the target site facilitates integration of the DNA of interest into the non-genic plant genomic sequence;
- and
- v. selecting transgenic cells comprising the DNA of interest integrated into the non-genic plant genomic sequence, wherein the non-genic plant genomic sequence is a corn genomic sequence selected from the group consisting of SEQ ID NOs:
- 123-172, 294, 299-551, 555 and 556 or a soybean genomic sequence selected from the group consisting of SEQ ID NOs:252-282 and 554.
-18. (canceled)
Dependencies are inferred from the claim text.