Liquid-phase hybrid capture method and liquid-phase hybrid capture kit

A liquid-phase hybrid capture method, comprising the following steps of:

• (1) probe design:
• designing a pool of probes based on nucleic acid target regions, wherein each probe comprises a probe binding sequence complementarily pairing with another probe, and a target specific sequence complementarily pairing with a nucleic acid target sequence, synthesizing each oligonucleotide based on corresponding sequence, and modifying a 5′ or 3′ end with a biomarker;
• (2) library construction;
• (3) hybrid capture
• i. configuring a hybridization system:
• wherein the hybridization system comprises the probes;
• and
• ii. carrying out a hybridization reaction:
• placing the hybridization system at 57-63° C. for hybridization for 1-2 h;
• (4) product capture:
• after the hybridization reaction is completed, adding streptavidin magnetic beads to the reaction system for hybrid capture;
• (5) product elution:
• after capture is completed, separately washing the captured product once with an elution buffer I, an elution buffer II and an elution buffer III;
• and
• (6) product amplification and purification:
• after the washing is completed, adding a PCR reaction system for a PCR amplification procedure, and after the reaction is completed, performing purification by using magnetic beads.