Engineered rubisco enzyme complexes
AssigneeCornell University
InventorsMyat T. LIN, Maureen HANSON
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Abstract
Provided herein are genetically engineered Rubisco enzymes and plants comprising the same. In one aspect, the disclosure features a genetically engineered plant comprising a Rubisco large subunit (LSU) comprising L2251 and K429Q amino acid substitution mutations, wherein the amino acid substitution mutations are numbered relative to the LSU of Nicotiana tabacum; and a Rubisco small subunit (SSU comprising N8G, V301, and E88Q amino acid substitution mutations, wherein the amino acid substitution mutations are numbered relative to the S-T2 subunit of Nicotiana tabacum.
Claims21
A genetically engineered plant comprising:
- (a) a Rubisco large subunit (LSU) comprising L225I and K429Q amino acid substitution mutations, wherein the amino acid substitution mutations are numbered relative to the LSU of Nicotiana tabacum (SEQ ID NO:
- 43);
- and
- (b) a Rubisco small subunit (SSU) comprising N8G, V30I, and E88Q amino acid substitution mutations, wherein the amino acid substitution mutations are numbered relative to the S-T2 subunit of Nicotiana tabacum (SEQ ID NO:
- 44).
A genetically engineered plant comprising:
- (a) a Rubisco LSU comprising V145I, L225I, and K429Q amino acid substitution mutations, wherein the amino acid substitution mutations are numbered relative to the LSU of Nicotiana tabacum (SEQ ID NO:
- 43);
- and
- (b) a Rubisco SSU comprising N8G, V30I, and E88Q amino acid substitution mutations, wherein the amino acid substitution mutations are numbered relative to the S-T2 subunit of Nicotiana tabacum (SEQ ID NO:
- 44).
A genetically engineered plant comprising:
- (a) a Rubisco LSU comprising L225I and K429Q amino acid substitution mutations, wherein the amino acid substitution mutations are numbered relative to the LSU of Nicotiana tabacum (SEQ ID NO:
- 43);
- and
- (b) a Rubisco SSU comprising N8G, K9M, E23D, R28K, V30I, and E88Q amino acid substitution mutations, wherein the amino acid substitution mutations are numbered relative to the S-T2 subunit of Nicotiana tabacum (SEQ ID NO:
- 44).
A genetically engineered plant comprising:
- (a) a Rubisco LSU comprising V91I, V145I, L225I, K429Q, E443D, C449S, V466R, A470E, V472M, and V474T amino acid substitution mutations, wherein the amino acid substitution mutations are numbered relative to the LSU of Nicotiana tabacum (SEQ ID NO:
- 43);
- and
- (b) a Rubisco SSU comprising N8G, K9M, S22T, E23D, R28K, V30I, N36K, N56H, E88Q, and Q96N amino acid substitution mutations, wherein the amino acid substitution mutations are numbered relative to the S-T2 subunit of Nicotiana tabacum (SEQ ID NO:
- 44).
-47. (canceled)
Dependencies are inferred from the claim text.