Culture method of fish germ stem cells
AssigneesKyushu University, National University Corporation, University of Miyazaki
InventorsTapas CHAKRABORTY, Kohei OHTA, Michiya MATSUYAMA, Sipra MOHAPATRA, Issei YAHIRO, Kodai MIZUMURA, Naoki NAGANO
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Abstract
The present invention provides a method for culturing a fish germ stem cell, including culturing the stem cell on a surface coated with vitronectin and in a medium containing the following 8 components: (1) insulin, (2) selenium, (3) transferrin, (4) L-ascorbic acid, (5) FGF2, (6) TGFβ, (7) NaHCO3 or KHCO3, (8) L-glutamine.
Claims20
A method for culturing a fish germ stem cell, comprising culturing the fish stem cell on a surface coated with vitronectin and in a medium comprising:
- (1) insulin,
- (2) selenium,
- (3) transferrin,
- (4) L-ascorbic acid,
- (5) FGF2,
- (6) TGFβ,
- (7) NaHCO3 or KHCO3,
- (8) L-glutamine.
A kit for culturing a fish germ stem cell, comprising vitronectin, and the following 8 components:
- (1) insulin,
- (2) selenium,
- (3) transferrin,
- (4) L-ascorbic acid,
- (5) FGF2,
- (6) TGFβ,
- (7) NaHCO3 or KHCO3,
- (8) L-glutamine.
Dependencies are inferred from the claim text.