Cell viability apparatus, system, and methods thereof
AssigneeO2M Technologies, LLC
InventorsMrignayani Kotecha, Boris Meerovich Epel, Eliyas Siddiqui, Safa Hameed
Life of this patent
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Abstract
A method includes placing a sample of cells, tissue, or an organ into an oxygen imaging system, circulating a humidified gas mixture around the tissue or organ, circulating conditioned air through the oxygen imaging system to maintain a temperature around the sample, and acquiring a three-dimensional oxygen map of the sample. The oxygen map provides a quantitative measure of cell viability and functionality.
Claims20
1-21. (canceled)
A method for non-destructively assessing in situ cell viability, the method comprising:
- placing a sample of cells, tissue, or an organ into an oxygen imaging system, the sample including an oxygen-sensitive EPR spin probe or EPR responsive contrast agent;
- circulating a humidified gas mixture around the sample;
- circulating conditioned air through the oxygen imaging system to maintain a temperature around the sample;
- and
- acquiring a three-dimensional oxygen map of the sample, wherein the oxygen map provides a quantitative measure of cell viability and functionality.
A method for non-destructively assessing in situ cell viability, the method comprising:
- placing a multi-well apparatus into an oxygen imaging system, wherein one or more wells include a sample of cells, tissue, or a three-dimensional scaffold;
- circulating a humidified gas mixture using a cover associated with the multi-well apparatus, the cover having a plurality of manifolds;
- circulating conditioned air through the oxygen imaging system to maintain a temperature within the multi-well apparatus;
- and
- acquiring a three-dimensional oxygen map of the sample in the one or more wells, wherein the oxygen map provides a quantitative measure of cell viability and functionality.
A method for non-destructively assessing in situ cell viability, the method comprising:
- placing a sample comprising a cell and scaffold system into an oxygen imaging system;
- circulating a humidified gas mixture around the sample;
- circulating conditioned air through the oxygen imaging system to maintain a temperature around the sample;
- and
- acquiring a three-dimensional oxygen map of the sample, the oxygen map corresponding to cell density of the sample.
Dependencies are inferred from the claim text.