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AssigneeThe University of Chicago
InventorsPatrick WILSON, Siriruk CHANGROB, Haley DUGAN, Christopher STAMPER
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Here, the inventors report that natural WT SARS-CoV-2 infection induces memory B cells expressing potently neutralizing antibodies against VOCs. Moreover, natural WT infection largely induced antibodies against spike epitopes outside of the RBD, most of which were non-neutralizing against WT and VOCs. Additionally. RBD-binding antibodies could be categorized into 3 distinct classes based on their binding profiles against RBD mutant constructs. The inventors identified VOC-neutralizing antibodies against three distinct regions of the spike protein, including the two epitopes on the RBD and one epitope in the NTD. Together, this study identifies that natural WT infection induces memory B cells that can produce neutralizing antibodies against recent SARS-CoV-2 VOCs and have the potential to be recalled by vaccination.
An antibody or antigen binding fragment comprising a heavy chain variable region and a light chain variable region:
An antibody or antigen binding fragment comprising a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region comprises a HCDR1, HCDR2, and HCDR3 having at least 80% sequence identity to the HCDR1, HCR2, HCR3 from a heavy chain variable region of a antibody clone of Table 1 and wherein the light chain variable region comprises a LCDR1, LCDR2, and LCDR3 having at least 80% sequence identity to the LCDR1, LCDR2, and LCDR3 from the light chain variable region of the same antibody clone of Table 1.
A nucleic acid encoding an antibody heavy chain, wherein the nucleic acid has at least 70% sequence identity to one of the nucleic acid sequences of a heavy chain of Table 2.
A nucleic acid encoding an antibody light chain, wherein the nucleic acid has at least 70% sequence identity to one of the nucleic acid sequences of a light chain of Table 2.
Dependencies are inferred from the claim text.