Mesenchymal stromal cell delivery via cardiopulmonary bypass provides neuroprotection
AssigneeChildren's National Medical Center
InventorNobuyuki ISHIBASHI
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Abstract
A method for improving neurobehavioral outcomes in neonates or infants undergoing cardiac bypass surgery by administering BM-MSCs, BM-MSC derived exosomes, or BM-MSC miRNA into CPB bypass circuit. Compositions comprising BM-MSCs or their products such as exosomes or miRNA are also aspects of the invention as is a kit suitable for performing the methods disclosed herein.
Claims20
A method for treating a neonatal subject who has congenital heart disease and who is undergoing cardiopulmonary bypass (CPB) surgery comprising administering bone marrow-derived mesenchymal stromal cells (BM-MSCs), BM-MSC derived exosomes, or miRNA found in BM-MSCs to said subject by delivery into a cardiopulmonary bypass circuit.
A method for diagnosing progression or recovery from CBP surgery comprising detecting miR-21-5p or other BM-MSC miRNAs in a subject and comparing their levels to those in a control subject.
A kit for detecting miR-21-5p comprising reagents for purifying, reverse transcribing, and/or amplifying miR-21-5p or other BM-MSC exosome-derived RNAs;
- wherein said reagents comprise QRT-PCR or TaqMan reagents suitable for detecting miR-21-5p.
Dependencies are inferred from the claim text.