Methods for nucleic acid detection
AssigneeAgricultural Genomics Institute At Shenzhen, Chinese Academy of Agricultural Sciences
InventorXinjie WANG
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Abstract
The present disclosure provides methods, kits, and materials for amplifying, enriching or detecting a target nucleic acid, especially in a small amount, in a sample by digesting or degrading non-target nucleic acids in the sample before or during amplification and may detect the target nucleic acids. Interference of the non-target nucleic acids before or during amplification or detection will be minimized and the sensitivity, accuracy and efficiency of the amplification or detection will be greatly increased.
Claims21
A method for amplifying, enriching or detecting target nucleic acids with alternation(s) of interest at a specified site in a sample, comprising digesting or degrading non-target nucleic acids without the alternation(s) of interest at the specified site in the sample by exposing the non-target nucleic acids to one or more proteins having an activity of cleaving nucleic acid that recognizes the base(s) at the specified site before or during amplification;
- wherein the alternation in the target nucleic acid includes deletion, substitution and/or insertion of one or more base(s) at the specified site as compared to the sequence of the non-target nucleic acid;
- wherein the method for detecting further comprises a step of detecting the amplified target nucleic acid after digesting or degrading.
(canceled)
A kit for amplifying or enriching or detecting target nucleic acids with alternation(s) of interest at a specified site in a sample, comprising reagents for amplification of target nucleic acids with alternation(s) of interest at the specified site in the sample and reagents for digesting non-target nucleic acids without the alternation(s) of interest at the specified site in the sample;
- wherein the alternation in the target nucleic acid includes deletion, substitution and/or insertion of one or more base(s) at the specified site as compared to the sequence of the non-target nucleic acid.
Dependencies are inferred from the claim text.