Plant regulatory elements and uses thereof
AssigneeMonsanto Technology LLC
InventorsStanislaw Flasinski, Barrett C. Foat, Mohammed Oufattole, Randall W. Shultz, Xiaoping Wei, Wei Wu, Shiaw-Pyng Yang
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Abstract
The invention provides DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. Transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.
Claims16
A DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
- a) a sequence with at least 85 percent sequence identity to any of SEQ ID NOS:
- 1-199, 211 and 212;
- b) a sequence comprising any of SEQ ID NOs:
- 1-199, 211 and 212;
- and
- c) a fragment of any of SEQ ID NOs:
- 1-199, 211 and 212 exhibiting gene-regulatory activity;
- wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.
A transgenic plant cell comprising a heterologous DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
- a) a sequence with at least 85 percent sequence identity to any of SEQ ID NOs:
- 1-199, 211 and 212;
- b) a sequence comprising any of SEQ ID NOs:
- 1-199, 211 and 212;
- and
- c) a fragment of any of SEQ ID NOs:
- 1-199, 211 and 212 exhibiting gene-regulatory activity;
- wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.
A transgenic plant, or part thereof, comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
- a) a sequence with at least 85 percent sequence identity to any of SEQ ID NOS:
- 1-199, 211 and 212;
- b) a sequence comprising any of SEQ ID NOs:
- 1-199, 211 and 212;
- and
- c) a fragment of any of SEQ ID NOs:
- 1-199, 211 and 212 exhibiting gene-regulatory activity;
- wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.
A transgenic seed comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
- a) a sequence with at least 85 percent sequence identity to any of SEQ ID NOs:
- 1-199, 211 and 212;
- b) a sequence comprising any of SEQ ID NOs:
- 1-199, 211 and 212;
- and
- c) a fragment of any of SEQ ID NOs:
- 1-199, 211 and 212 exhibiting gene-regulatory activity;
- wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.
A method of producing a commodity product comprising:
- a) obtaining a transgenic plant or part thereof comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
- 1) a sequence with at least 85 percent sequence identity to any of SEQ ID NOs:
- 1-199, 211 and 212;
- 2) a sequence comprising any of SEQ ID NOs:
- 1-199, 211 and 212;
- and
- 3) a fragment of any of SEQ ID NOs:
- 1-199, 211 and 212 exhibiting gene-regulatory activity;
- wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule;
- and
- b) producing the commodity product therefrom.
A commodity product comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
- a) a sequence with at least 85 percent sequence identity to any of SEQ ID NOs:
- 1-199, 211 and 212;
- b) a sequence comprising any of SEQ ID NOs:
- 1-199, 211 and 212;
- and
- c) a fragment of any of SEQ ID NOs:
- 1-199, 211 and 212 exhibiting gene-regulatory activity;
- wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.
A method of expressing a transcribable polynucleotide molecule comprising:
- a) obtaining a transgenic plant comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
- 1) a sequence with at least 85 percent sequence identity to any of SEQ ID NOs:
- 1-199, 211 and 212;
- 2) a sequence comprising any of SEQ ID NOs:
- 1-199, 211 and 212;
- and
- 3) a fragment of any of SEQ ID NOs:
- 1-199, 211 and 212 exhibiting gene-regulatory activity;
- wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule;
- and
- b) cultivating said transgenic plant, wherein the transcribable polynucleotide is expressed.
Dependencies are inferred from the claim text.