Rna export for measurement and manipulation of cells

A pharmaceutical composition comprising a population of LNs (i) derived from the expression of a nucleic acid composition comprising:

• one or more first polynucleotide(s) encoding an RNA exporter protein;
• one or more second polynucleotide(s) each encoding one or more cargo RNA molecule(s);
• one or more third polynucleotide(s) encoding a fusogen;
• and
• one or more fourth polynucleotide(s) encoding an export modulator;
• wherein the RNA exporter protein comprises:
• an RNA-binding domain,
• a membrane-binding domain, and
• an interaction domain capable of nucleating self-assembly;
• or
• (ii) secreted by a population of sender cells comprising:
• one or more first polynucleotide(s) encoding an RNA exporter protein;
• one or more second polynucleotide(s) each encoding one or more cargo RNA molecule(s);
• one or more third polynucleotide(s) encoding a fusogen;
• and
• one or more fourth polynucleotide(s) encoding an export modulator;
• wherein the RNA exporter protein comprises:
• an RNA-binding domain,
• a membrane-binding domain, and
• an interaction domain capable of nucleating self-assembly,
• wherein the pharmaceutical composition further comprises one or more pharmaceutically acceptable carriers, diluents and/or excipients.

A method of treating or preventing a disease or disorder in a subject in need thereof, comprising:

• administering to the subject an effective amount of a pharmaceutical composition of comprising a population of LNs (i) derived from the expression of a nucleic acid composition comprising:
• one or more first polynucleotide(s) encoding an RNA exporter protein;
• one or more second polynucleotide(s) each encoding one or more cargo RNA molecule(s);
• one or more third polynucleotide(s) encoding a fusogen;
• and
• one or more fourth polynucleotide(s) encoding an export modulator;
• wherein the RNA exporter protein comprises:
• an RNA-binding domain,
• a membrane-binding domain, and
• an interaction domain capable of nucleating self-assembly;
• or
• (ii) secreted by a population of sender cells comprising:
• one or more first polynucleotide(s) encoding an RNA exporter protein;
• one or more second polynucleotide(s) each encoding one or more cargo RNA molecule(s);
• one or more third polynucleotide(s) encoding a fusogen;
• and
• one or more fourth polynucleotide(s) encoding an export modulator:
• wherein the RNA exporter protein comprises:
• an RNA-binding domain,
• a membrane-binding domain, and
• an interaction domain capable of nucleating self-assembly and one or more pharmaceutically acceptable carriers, diluents and/or excipients, thereby treating or preventing the disease or disorder in the subject.