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US 2025/0019674 A1Published application
Protein degradation system, components, and methods
AssigneeNorth Carolina State University
InventorsMarcela Pierce, Linzhou Huang
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Abstract
The present disclosure provides components and systems for targeted protein degradation in cells (e.g., in plant cells). In particular, the present disclosure provides a protein containing the Leucine-Rich Repeat (LRR) and novel E3 ligase (NEL) domains of SspH1 for use with Homology Region 1b (HR1b) domain of human PKN1 for degrading target proteins.
Claims20
A system for targeted protein degradation in a cell comprising:
- a first polypeptide comprising Leucine-Rich Repeat (LRR) domain and novel E3 ligase (NEL) catalytic domain of SspH1, or a nucleic acid encoding thereof;
- and
- a second polypeptide comprising Homology Region 1b (HR1b) domain of human PKN1, or a nucleic acid encoding thereof.
A fusion protein comprising:
- a protein of interest;
- and
- Homology Region 1b (HR1b) domain of human PKN1 or a polypeptide comprising Leucine-Rich Repeat (LRR) domain and novel E3 ligase (NEL) catalytic domain of SspH1.
Dependencies are inferred from the claim text.