Compositions and methods for protein expression with rna
AssigneeExcepgen Inc.
InventorsBarbara Mertins, Thomas Folliard, Imre Mäger, Jason Wojcechowskyj
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Abstract
The compositions and methods provided herein include a ribonucleic acid (RNA) encoding a nuclear cytoplasmic transport (NCT) inhibitor protein to improve target protein expression, e.g., a target protein encoded by a DNA vector, a messenger RNA, a self-amplifying RNA, or an RNA comprising an unmodified uridine nucleotide. The compositions and methods provided herein may be used to improve the expression of any target protein, for example a viral protein antigen for use in a vaccine.
Claims21
-29. (canceled)
A method of reducing cell stress signals elicited by exposure to exogenous polynucleotides, the method comprising:
- introducing a polynucleotide encoding a target protein into a cell;
- and
- introducing a ribonucleic acid (RNA) encoding a nucleocytoplasmic transport (NCT) inhibitor protein into the cell, wherein expression of the NCT inhibitor protein reduces a cellular stress response associated with the introduction of the polynucleotide into the cell, thereby enhancing expression of the target protein.
Dependencies are inferred from the claim text.