Anti-cd40 single-chain variable fragment and human il-21 fusion protein (cd40scfv-il-21)
AssigneeWisconsin Alumni Research Foundation
InventorsJacques GALIPEAU, Andrea PENNATI, Shala YUAN
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Abstract
The present invention provides fusion polypeptides comprising an anti-CD40 scFv and IL-21. Also provided are methods for making and using the fusion polypeptides for the generation of B regulatory cells (Bregs), and methods of treatment using said Bregs.
Claims35
A polypeptide comprising from N to C terminus:
- an scFv specific for CD40 linked to IL-21.
The polypeptide of any one of the preceding claims, wherein the scFv is linked via a peptide bond to IL-21.
The polypeptide of any one of the preceding claims, wherein the scFv is linked to IL-21 via a linker peptide.
The polypeptide of any one of the preceding claims, wherein the polypeptide further comprises a signal peptide.
The polypeptide of any one of the preceding claims, wherein IL-21 comprises SEQ ID NO:
- 10 or has 90% identity with SEQ ID NO:
- 10.
The polypeptide of any one of the preceding claims, wherein the polypeptide comprises SEQ ID NO:
- 11 or has 90% identity with SEQ ID NO:
- 11.
A kit, system, or platform comprising a polypeptide comprising from N to C terminus:
- an scFv specific for CD40 linked to IL-21, and a TLR9 agonist.
Dependencies are inferred from the claim text.