Stable production systems for aav vector production
AssigneeAsimov Inc.
InventorsChristopher S. Stach, Alec A.K. Nielsen
Life of this patent
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Abstract
Described herein are AAV vector production systems and HSV-helper systems. Also described herein are engineered cells and kits comprising an AAV vector production system and/or an HSV-helper system and methods of using the same for AAV vector production.
Claims153
An engineered cell for AAV production, comprising one or more stably integrated polynucleic acid collectively comprising a nucleic acid sequence encoding for each of:
- Rep52 or Rep40;
- Rep78 or Rep68;
- E2A;
- E4Orf6;
- VARNA;
- VP1;
- VP2;
- VP3;
- and AAP;
- at least one of which is operably linked to a chemically inducible promoter;
- wherein:
- (i) the nucleic acid sequence encoding for Rep68 lacks the Rep52 start codon;
- (ii) the nucleic acid sequence encoding for Rep78 lacks the Rep52 start codon and the Rep68/40 splice site;
- (iii) the nucleic acid sequence encoding for Rep52 lacks the Rep40 splice site;
- and
- (iv) the nucleic acid sequence encoding for VP1 lacks start codons for VP2, VP3, and AAP.
An engineered cell for adeno-associated virus (AAV) production, comprising one or more stably integrated polynucleic acids collectively comprising a nucleic acid sequence encoding for each of:
- Rep52 or Rep40;
- Rep78 or Rep68;
- E2A;
- E4Orf6;
- VARNA;
- VP1;
- VP2;
- VP3;
- and AAP;
- each of which is operably linked to a chemically inducible promoter.
An engineered cell for AAV production, comprising one or more stably integrated polynucleic acid collectively comprising a nucleic acid sequence encoding for each of:
- UL5;
- UL8;
- UL29;
- UL30;
- UL42;
- and UL52;
- each of which is operably linked to a chemically inducible promoter.
Dependencies are inferred from the claim text.