Method of evaluating fibrinolytic resistance activity in blood plasma

A method of evaluating fibrinolytic resistance activity in blood plasma of a test animal, the method comprising:

• a pretreatment step of adding an anionic surfactant to some of a blood plasma sample derived from the test animal and incubating the blood plasma sample for a predetermined time;
• a first fibrinolytic resistance measurement step of measuring an index value of fibrinolytic resistance activity by PAI-1 and α2AP in the treated blood plasma sample obtained by the pretreatment step;
• a second fibrinolytic resistance measurement step of measuring an index value of fibrinolytic resistance activity by PAI-1 and α2AP in some of the blood plasma sample derived from the test animal;
• and
• an evaluation step of evaluating the fibrinolytic resistance activity by PAI-1 and α2AP in the blood plasma of the test animal, based on the index value measured in the first fibrinolytic resistance measurement step and the index value of untreated blood plasma measured in the second fibrinolytic resistance measurement step,
• wherein the index value is the time until a plasma clot formed by thrombin in the presence of a nonionic surfactant and a plasminogen activator is lysed, or a plasmin generation rate in the case of adding thrombin in the presence of a nonionic surfactant and a plasminogen activator.