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AssigneeReneuron Limited
InventorsPaul HOLE, Ruben DEOGRACIAS, Randolph CORTELING
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The present invention provides proteins that are linked to glycosylphosphatidylinositol (GPI). The invention provides a fusion protein comprising a protein of interest and a carboxy-terminal glycosylphosphatidylinositol (GPI) anchor peptide sequence. Proteins of interest include neurotrophins such as BDNF. The invention also provides a fusion protein comprising a protein of interest, a linker peptide and a final carboxy-terminal residue, wherein the final carboxy-terminal residue is covalently linked to glycosylphosphatidylinositol (GPI). The GPI may be anchored to the membrane of an extracellular vesicle. Methods of making and using the fusion proteins and extracellular vesicles of the invention are also provided, including their use in therapy.
A fusion protein comprising a protein of interest and a carboxy-terminal glycosylphosphatidylinositol (GPI) anchor peptide sequence, optionally wherein the protein of interest and GPI anchor protein are separated by a linker peptide.
A fusion protein comprising a protein of interest, a linker peptide and a final carboxy-terminal residue, wherein the final carboxy-terminal residue is covalently linked to glycosylphosphatidylinositol (GPI).
A fusion protein according to any preceding claim, wherein the protein of interest is:
A fusion protein according to any preceding claim, wherein the protein of interest is Brain-Derived Neurotrophic Factor (BDNF).
A fusion protein according to any preceding claim, wherein the protein of interest comprises a mature sequence and optionally a pro-peptide and/or signal sequence.
A fusion protein according to any preceding claim, in the form of a biologically-active homodimer.
A fusion protein according to any preceding claim, comprising a purification tag, optionally wherein the purification tag comprises or consists of the sequence YPYDVPDYA or a functional variant thereof.
A fusion protein according to any preceding claim, comprising:
A polynucleotide encoding a fusion protein according to any preceding claim.
Dependencies are inferred from the claim text.