Novel markers combination in sweet basil for disease resistances

A method for producing a disease resistant cultivated basil plant and/or seed, the method comprising:

• a. selecting a first Ocimum americanum basil plant exhibiting resistance or tolerance to basil downy mildew (BDM) race-0 and comprising a haplotype associated with the BDM race-0 resistance or tolerance;
• and/or
• selecting a second Ocimum americanum basil plant exhibiting resistance or tolerance to basil downy mildew (BDM) race-1 and comprising a haplotype associated with the BDM race 1 resistance or tolerance;
• b. crossing the first and/or the second BDM resistant or tolerant basil plant with a basil plant lacking BDM resistance or tolerance, to produce F1 basil progeny plants;
• c. optionally backcrossing the F1 plants with the BDM nonresistant basil plant;
• and
• d. selecting a basil plant having resistance to BDM race-0 and/or BDM race-1, wherein the BDM resistant or tolerant basil plant has introgressed into its genome the haplotype associated with the resistance to BDM race-0 and/or race-1,
• wherein said BDM resistance or tolerance associated haplotype comprises at least one of:
• a single nucleotide polymorphism (SNP) of nucleotide T at position 7 of a sequence comprising at least 80% identity to SEQ ID NO:1, SNP of nucleotide A at position 5 of a sequence comprising at least 80% identity to SEQ ID NO:2, SNP of nucleotide C at position 48 of a sequence comprising at least 80% identity to SEQ ID NO:3, SNP of nucleotide G at position 4 of a sequence comprising at least 80% identity to SEQ ID NO:4, SNP of nucleotide T at position 19 of a sequence comprising at least 80% identity to SEQ ID NO:5, SNP of nucleotide A at position 8 of a sequence comprising at least 80% identity to SEQ ID NO:6, SNP of nucleotide T at position 29 of a sequence comprising at least 80% identity to SEQ ID NO:7, SNP of nucleotide T at position 55 of a sequence comprising at least 80% identity to SEQ ID NO:8, SNP of nucleotide T at position 41 of a sequence comprising at least 80% identity to SEQ ID NO:9, SNP of nucleotide C at position 11 of a sequence comprising at least 80% identity to SEQ ID NO:10, SNP of nucleotide G at position 6 of a sequence comprising at least 80% identity to SEQ ID NO:11 or any combination thereof.

An allele, haplotype, molecular marker or genetic determinant being inherited to progeny plant, wherein said allele, haplotype, molecular marker or genetic determinant is associated with resistance or tolerance to BDM race-0 or to BDM race-1, wherein said allele, haplotype, molecular marker or genetic determinant is selected from:

• a. a single nucleotide polymorphism (SNP) of nucleotide T at position 7 of a sequence comprising at least 80% identity to SEQ ID NO:1, SNP of nucleotide A at position 5 of a sequence comprising at least 80% identity to SEQ ID NO:2, SNP of nucleotide C at position 48 of a sequence comprising at least 80% identity to SEQ ID NO:3, SNP of nucleotide G at position 4 of a sequence comprising at least 80% identity to SEQ ID NO:4, SNP of nucleotide T at position 19 of a sequence comprising at least 80% identity to SEQ ID NO:5, SNP of nucleotide A at position 8 of a sequence comprising at least 80% identity to SEQ ID NO:6, SNP of nucleotide T at position 29 of a sequence comprising at least 80% identity to SEQ ID NO:7, or any combination thereof, associated with resistance to BDM race-0;
• b. SNP of nucleotide T at position 55 of a sequence comprising at least 80% identity to SEQ ID NO:8, SNP of nucleotide T at position 41 of a sequence comprising at least 80% identity to SEQ ID NO:9, SNP of nucleotide C at position 11 of a sequence comprising at least 80% identity to SEQ ID NO:10, SNP of nucleotide G at position 6 of a sequence comprising at least 80% identity to SEQ ID NO:11 or any combination thereof, associated with resistance to BDM race-1;
• and
• c. any combination thereof.