Heparosan-producing recombinant cells
AssigneeGivaudan SA
InventorsDominique LOUIS, Karine JAILLARDON, Muriel MERKAMM, Dominique THOMAS
Life of this patent
Estimating the term…
Abstract
The present invention relates to the field of bio-production of heparosan. There is a need in the art for heparosan production methods allowing its highly efficient synthesis and secretion. The solution proposed in the present invention is the use of a genetically modified cell comprising many modifications as described in the present text. The present invention further proposes methods allowing the bio-production of heparosan having a controlled molecular weight using the genetically modified cells of the invention.
Claims30
A recombinant host cell (i) or a recombinant yeast cell (ii) producing heparosan wherein the recombinant host cell (i) and the recombinant yeast cell (ii) comprise:
- (a) one or more recombinant nucleic acids encoding a polypeptide having a heparosan synthase (HSS) activity;
- (b) one or more recombinant nucleic acids encoding a polypeptide having a UDP-Glucose dehydrogenase (UDP-GlcDH or HASB) activity,
- wherein:
- the recombinant host cell (i) further comprises (c) one or more recombinant nucleic acids encoding a polypeptide having a glucuronosyl-disulfoglucosamine glucuronidase activity, and
- the recombinant yeast cell (ii) optionally further comprises (c) one or more recombinant nucleic acids encoding a polypeptide having a glucuronosyl-disulfoglucosamine glucuronidase activity,
- wherein the polypeptide having a glucuronosyl-disulfoglucosamine glucuronidase activity comprises a secretion signal, and optionally an anchoring signal, heparosan is produced by the recombinant host cell (i) or the recombinant yeast cell (ii).
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.