Methods and compositions for reducing smoke taint in fermented beverages
AssigneeBerkeley Fermentation Science Inc.
InventorsRachel Li, Nicholas Harris, Charles Denby, Jeremy Roop, Daniel Liu, Francis Cunningham
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Abstract
Provided herein are genetically modified yeast cells that recombinantly express a glycosidase enzyme. Provided herein are genetically modified yeast cells that recombinantly express a glycosidase enzyme and/or an O-methyltransferase enzyme. Also provided are methods of producing fermented beverages and compositions comprising ethanol using the genetically modified yeast cells described herein.
Claims145
A method of producing a fermented product, the method comprising:
- contacting a genetically modified yeast cell (modified cell) with a medium comprising at least one fermentable sugar and one or more non-volatile phenolic glycosides associated with smoke taint, thereby hydrolyzing the one or more non-volatile phenolic glycosides to produce one or more volatile phenols;
- wherein the genetically modified cell comprises a heterologous gene encoding an enzyme having glycosidase activity.
The method of 23, wherein the second heterologous gene encoding an enzyme having O-methyltransferase activity is derived from Silene latifolia, Solanuma lycopersicum, Rosa hybrida, Ocimum basilicum, Eriobotrya japonica, Pinus taeda, Fragaria ananassa, Schizosaccharomyces pombe, or Zinnia elegans.
A genetically modified yeast cell (modified cell) comprising:
- (i) a first heterologous gene encoding an enzyme having glycosidase activity, and
- (ii) a second heterologous gene encoding an enzyme having O-methyltransferase activity.
A method of producing a fermented product, the method comprising:
- contacting a genetically modified yeast cell (modified cell) with a medium comprising at least one fermentable sugar and one or more volatile phenols, thereby converting the one or more volatile phenols to one or more methylated volatile phenols;
- wherein the genetically modified cell comprises a heterologous gene encoding an enzyme having O-methyltransferase activity.
The method of claim of 107 or 108, wherein the enzyme having glycosidase activity is a glucosidase or a rhamnosidase.
Dependencies are inferred from the claim text.