Device and method for manipulation of extracellular vesicles

A method for loading an extracellular vesicle with a cargo molecule selected from the group comprising a pharmaceutical drug, a protein, a nucleic acid, a dye molecule, comprising the steps:

• a. providing an aqueous medium comprising an extracellular vesicle, wherein the aqueous medium is in contact with a surface that is electrically conductive,
• wherein the aqueous medium is a loading solution comprises a cargo molecule;
• b. applying a loading voltage to the surface;
• characterized in that
• said surface is an electrically conductive micro- or nanostructured material.

A device for isolating of a biomolecule, or for isolating and/or loading of an extracellular vesicle, said device comprising a chamber configured for receiving a liquid sample, wherein

• a first surface of said chamber is formed by a high-surface, electrochemically active material embedded in a non-electrically conductive polymer matrix, said first surface forming a working electrode (2);
• said device comprises a counter electrode (1) configured to contact said liquid sample;
• a first (9) and second (7) electrically conductive connection connectable to a voltage source is connected to said working and counter electrode, respectively;
• wherein the high-surface, electrochemically active material is a carbon fibre (CF) microelectrode, and wherein the CF microelectrode is embedded in a polymer matrix, and an exposed portion of the conductive micro- or nanostructured material is exposed to said liquid sample.