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US 2024/0400983 A1Published application
Cell aggregate including pituitary hormone-producing cells and method for producing same
AssigneesSumitomo Pharma Co., Ltd., National University Corporation Tokai National Higher Education and Research System, Sumitomo Chemical Company, Limited
InventorsShiori TAGA, Atsushi KUWAHARA, Hidetaka SUGA, Tokushige NAKANO
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Abstract
The present invention provides a method for efficiently producing a cell aggregate containing pituitary hormone-producing cells from pluripotent stem cells.
Claims19
A cell aggregate containing a pituitary hormone-producing cell, wherein
- (1) the percentage of cells positive for at least one of EpCAM and E-cadherin in the cell aggregate is not less than 80%,
- (2) the percentage of neural cells in the cell aggregate is not more than 20%;
- (3) the percentage of ACTH-positive cells in the cell aggregate is not less than 5%;
- (4) the cell density of the cell aggregate is 3,000 to 20,000 cells/mm2,
- (5) the major axis of the cell aggregate is 200 to 3,000 μm,
- (6) on the surface of the cell aggregate, cells positive for at least one of EpCAM and E-cadherin form a plane attachment to each other to form an epithelial structure, and
- (7) the cell aggregate forms a sponge-like structure in the inside.
4 more elements
A method for producing a cell aggregate containing a pituitary hormone-producing cell, including the following steps:
- (1) a step of separating a cell expressing EpCAM from a dispersed cell population derived from a pluripotent stem cell and including a hypothalamic cell, a pituitary progenitor cell, and a pituitary hormone-producing cell, and
- (2) a step of suspension culturing the separated EpCAM-expressing cell for not less than 14 days under conditions that allow the pituitary progenitor cell to mature into a pituitary hormone-producing cell.
Dependencies are inferred from the claim text.