Materials for valuable metal recovery
OwnerDavid L. Kaplan, Huan-Hsuan Hsu +2inventors
InventorsDavid L. Kaplan, Huan-Hsuan Hsu, Ryan Scheel, Xiaocheng Jiang
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Abstract
Capture membranes for lanthanide metal ions can be made from fusion proteins having at least one lanthanide metal binding sequence (SEQ ID NO: 1-4) covalently bound to a silk-elastin-like polymer (SELP). Capture membranes can be made from silk nanofibrils that are surface-modified with a lanthanide metal binding molecule. The capture membranes can have a layered structure or can contained cross-linked peptides in a hydrogel.
Claims20
A recombinant fusion protein comprising at least one lanthanide metal binding sequence (SEQ ID NO:
- 1-4) covalently bound to a silk-elastin-like polymer (SELP), wherein the recombinant fusion protein exhibits an apparent or true binding affinity towards a lanthanide metal ion of between 2.0×105 M−1 and 1.8×107 M−1 or higher.
A modified silk nanofibril wherein a lanthanide metal binding molecule is coupled to a surface of a silk nanofibril, wherein the lanthanide metal binding molecule comprises at least one lanthanide metal binding sequence (SEQ ID NO:
- 1-4).
A modified silk protein comprising:
- (i) at least one modified lanthanide metal-binding protein according to SEQ ID NO:
- 1-4, or a variant thereof which differs in each case from said sequence by 1 amino acid substitution, 1 to 2 amino acid deletion(s), and/or 1 to 2 amino acid insertion(s);
- and
- (ii) at least one modified silk-elastin-like polymer (SELP), wherein the SELP comprises the sequence ((GAGAGS)a(GXGVP)b)c, wherein a ratio of a to b is between 1:3 and 1:5, wherein a is 1, 2, or 3, wherein c is between 5 and 20, and wherein X is valine, cysteine, or tyrosine, or a variant thereof which differs in each case from said sequence by 1 amino acid substitution, 1 to 2 amino acid deletion(s), and/or 1 to 2 amino acid insertion(s).
Dependencies are inferred from the claim text.