Back to results
US 2024/0393343 A1Published application
Methods and materials for large-scale assessment of ligand binding selectivity of g-quadruplex recognition using custom g4 microarrays
OwnerDanzhou Yang, Guanhui Wu +1inventors
InventorsDanzhou Yang, Guanhui Wu, Charles Vinson
Life of this patent
Estimating the term…
Abstract
Described herein are devices and processes using single-stranded DNA sequences capable of forming G-quadruplexes (G4s) to assess the binding affinity and binding selectivity of potential G4-interactive ligands.
Claims20
A method for determining binding preferences of a non-fluorescent test compound for one or more target G-quadruplex moieties, the method comprising;
- a) incubating a device comprising a plurality of single-stranded nucleic acid molecules capable of forming one or more G-quadruplex moieties including the target G-quadruplex moieties with a solution comprising a G-quadruplex stabilizing cation selected from the group consisting of Na+ and K+;
- b) incubating the device with a solution of a compound capable of providing a fluorescent signal (a fluorescent compound), wherein the fluorescent compound is capable of binding to the target G-quadruplex moieties;
- c) measuring a first fluorescent signal from the fluorescent compound bound to the device;
- d) removing the fluorescent compound from the device;
- e) contacting the device with a solution of the fluorescent compound and the test compound;
- f) measuring a second fluorescent signal from the fluorescent compound bound to the device;
- and
- g) using the first fluorescent signal and the second fluorescent signal to calculate the binding preferences of the test compound.
5 more elements
A method for determining the binding preference of a test compound capable of providing a fluorescent signal (a fluorescent test compound) for one or more target G-quadruplex moieties, the method comprising the steps of;
- a) incubating a device comprising a plurality of single-stranded nucleic acid molecules capable of forming one or more G-quadruplex moieties including the target G-quadruplex moieties with a solution comprising a G-quadruplex stabilizing cation selected from the group consisting of Na+ and K+;
- b) contacting the fluorescent test compound with the device;
- c) measuring a first fluorescent signal from the fluorescent test compound bound to the device;
- d) incubating the device with a solution of solution of Li+;
- e) contacting the fluorescent test compound with the device;
- f) measuring a second fluorescent signal from the fluorescent test compound bound to the device;
- g) using the first fluorescent signal and the second fluorescent signal to calculate the binding preference of the fluorescent test compound.
4 more elements
Dependencies are inferred from the claim text.