Microfluidic Magnetic Separation Device with A Magnetophoretic Gradient for Isolation of Target Cell Populations from Fluid Samples

A microfluidic magnetic device for isolation of one or more types of target cells from a blood sample, the device comprising:

• a) one or more inlets to receive a blood sample;
• b) one or more deterministic lateral displacement (DLD) channels connected to the inlets, wherein the one or more DLD channels separate white blood cells from red blood cells in the blood sample;
• c) one or more mixers connected to the one or more DLD channels, wherein the DLD channels mix magnetic nanoparticles (MNPs) and one or more negative selection binding agents with the separated white blood cells, wherein the one or more negative selection binding agents selectively bind to one or more surface markers on non-target cells, wherein the one or more surface markers are not present on the one or more types of target cells;
• d) one or more incubation tubings connected to the one or more mixers, wherein the white blood cells are incubated with the MNPs and the one or more negative selection binding agents in the one or more incubation tubings, wherein the non-target cells that are bound to the one or more negative selection binding agents are selectively magnetically labeled with the MNPs;
• e) a magnetophoretic separation device (MSD) connected to the one or more incubation tubings, wherein the MSD applies an exponentially increasing magnetophoretic gradient to capture the magnetically labeled non-target cells;
• and
• f) one or more outlets connected to the MSD, wherein the one or more types of target cells are expelled from the one or more outlets.