Apparatus for localizing single fluorescent molecules comprised in a sample using single molecule localization microscopy

Abstract

An apparatus for localizing single fluorescent molecules comprised in a sample using single molecule localization microscopy. The apparatus comprises a scanning unit configured to scan the sample using a light beam at a first wavelength having a predetermined irradiance, an image acquisition unit configured to acquire a first image and a second image at a second wavelength, a localization unit configured to localize the single fluorescent molecules in the second image. Additionally, the apparatus comprises a determination unit configured to determine, based on the first image, one or more local densities of the single fluorescent molecules; and a calculation unit configured to calculate one or more local irradiances of the light beam as a predetermined function of each one of the one or more local densities of the single fluorescent molecules. The scanning unit is further configured to scan the sample using the one or more local irradiances of the light beam.

Dependencies are inferred from the claim text.