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AssigneeGenzyme Corporation
InventorsCatherine R. O'RIORDAN, Brenda BURNHAM
Estimating the term…
Provided herein are methods to characterize preparations of recombinant viral particles using analytical ultracentrifugation. Recombinant viral particles include recombinant adeno-associated viral particles, recombinant adenoviral particles, recombinant lentiviral particles and recombinant herpes simplex virus particles. Variant species of recombinant viral particles including empty capsids and recombinant viral particles with variant genomes (e.g., truncated genomes, aggregates, recombinants) can be identified and quantitated. The methods can be used to characterize preparations of recombinant viral particles regardless of the sequence of the recombinant viral genome or the serotype of the recombinant viral capsid.
A method of characterizing a preparation of recombinant viral particles comprising the steps of
A method to assess vector genome integrity of recombinant viral particles in a preparation of recombinant viral particles comprising
A method to determine the presence of empty capsids or capsid particles comprising variant sized recombinant viral genomes in a preparation of recombinant viral particles comprising the steps of
A method of measuring the relative amount empty capsids in a preparation of recombinant viral particles comprising the steps of
A method of measuring the relative amount of capsid particles comprising variant recombinant viral genomes or empty viral capsid particles in a preparation of recombinant viral particles comprising the steps of
A method of measuring the relative amount of capsid particles comprising variant recombinant viral genomes in a preparation of recombinant viral particles comprising the steps of
A method of measuring the relative amount of recombinant viral particles comprising intact viral genomes in a preparation of recombinant viral particles comprising the steps of
A method of determining the heterogeneity of recombinant viral particles in a preparation of recombinant viral particles comprising the steps of
The method of claim any one of 48-54, wherein the frictional ratio is about 1.0.
The method of any one of the preceding claims wherein the recombinant viral particles have been purified using one or more purification steps.
Dependencies are inferred from the claim text.