Dna based identification of seafood species in samples

A method for identifying seafood species of different origin and processing degree in a food sample, comprising the steps of:

• a) isolating DNA from the sample,
• b) amplifying fragments of said DNA with at least 2 primer sets (ps) selected from the group of
• i. ps 1 for identifying seafood species of the family of Crustaceans comprising one or more primer pairs of one forward primer selected from any one of SEQ ID NOs:
• 1 and 2, and a corresponding reverse primer SEQ ID NO:
• 15 and/or reverse complement sequences of the primer sequences;
• ii. ps 2 for identifying seafood species of the family of Cephalopods comprising one or more primer pairs of one forward primer selected from any one of SEQ ID NOs:
• 3 to 5, and a corresponding reverse primer SEQ ID NO:
• 16 and/or reverse complement sequences of the primer sequences;
• iii. ps 3 for identifying seafood species of the family of Gastropoda comprising one or more primer pairs of forward primer SEQ ID NO:
• 6, and one reverse primer selected from any one of SEQ ID NOs:
• 17 to 18 and/or reverse complement sequences of the primer sequences;
• iv. ps 4 for identifying seafood species of the family of Veneridae comprising one or more primer pairs of one forward primer selected from any one of SEQ ID NOs:
• 7 to 11, and one reverse primer selected from any one of SEQ ID NOs:
• 19 to 21 and/or reverse complement sequences of the primer sequences;
• v. ps 5 for identifying seafood species of the family of Ostreidae comprising the forward primer SEQ ID NO:
• 12 and a corresponding reverse primer SEQ ID NO:
• 22 and/or reverse complement sequences of the primer sequences;
• vi. ps 6 for identifying seafood species of the family of Pectinidae comprising the forward primer SEQ ID NO:
• 13 and a corresponding reverse primer SEQ ID NO:
• 23 and/or reverse complement sequences of the primer sequences;
• and
• vii. ps 7 for identifying seafood species of the family of Mytilidae comprising one or more primer pairs of forward primer SEQ ID NO:
• 14, and one reverse primer selected from any one of SEQ ID NOs:
• 24 to 25 and/or reverse complement sequences of the primer sequences,
• c) sequencing the amplified DNA fragments of step b), and
• d) identifying the seafood species by comparison of the sequences obtained by steps a) to c) with reference sequences of seafood species.