Solution for the preservation of cells
AssigneeAnacyte Laboratories GmbH
InventorHAUKE SIMON GÜNTHER
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Abstract
The invention refers to a solution for the conservation or preservation of cells in blood samples or other body fluids, in cell culture or tissue samples. The solution contains a cell membrane permeable proton carrier and is essentially free of chaotropic substances, alcohols and detergents. Furthermore, the solution according to the present invention has a pH value which produces an intracellular pH of the cells of less than 6. Such a solution is advantageously used in sample tubes, blood collection syringes or blood collection tubes.
Claims20
Use of a solution for the conservation, preservation or/and fixation of at least one eukaryotic cell, in particular of tumor cells and circulating tumor cells, in a blood sample or another body fluid, or of cells from cell culture or of cells in tissue samples,
- characterized in that
- it is an aqueous solution which
- a) contains a cell-permeable proton carrier, wherein the proton carrier is selected from C2-C5 carboxylic acids or a mixture of such carboxylic acids, or other acids which are membrane-permeable in protonated form and is present as an acid/base system, and forms a pH value in the acidic range.
- b) is free of such amounts of chaotropic substances, alcohols or detergents, which destroy morphological integrity of the cells,
- c) maintains the morphological integrity of the cells, and
- d) has a pH value that generates an intracellular pH of the cells of less than 6.
A sample tube, blood collection syringe or blood collection tube, which are characterized in that they contain a solution for the conservation, preservation or/and fixation of at least one eukaryotic cell, in particular of tumor cells and circulating tumor cells, in a blood sample or another body fluid, or of cells from cell culture or of cells in tissue samples, which
- a) contains a cell-permeable proton carrier, wherein the proton carrier is selected from C2-C5 carboxylic acids or a mixture of such carboxylic acids, or other acids which are membrane-permeable in protonated form and is present as an acid/base system, and forms a pH value in the acidic range.
- b) is free of such amounts of chaotropic substances, alcohols or detergents, which destroy the morphological integrity of the cells,
- c) maintains the morphological integrity of the cells, and
- d) has a pH value of less than 6 that generates an intracellular pH of the cells of less than 6.
Dependencies are inferred from the claim text.