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US 2024/0392333 A1Published application
Methods and compositions for increasing glucose yield from grain
AssigneeGreenlab, Inc.
InventorsElizabeth E. Hood, John A. Howard
Life of this patent
Estimating the term…
Abstract
The present disclosure provides methods of converting cellulose in grain to glucose for ethanol production. By providing cost-effective cellulases expressed in the grain, the methods increase glucose yield compared to the use of starch degrading amylase enzymes alone.
Claims40
A method of producing glucose, the method comprising:
- contacting milled grain comprising an effective amount of a heterologous cellulase with an enzyme composition comprising β-glucosidase under conditions sufficient to hydrolyze cellulose in the milled grain to glucose.
A method of producing ethanol, the method comprising:
- contacting milled grain comprising an effective amount of a heterologous cellulase with an enzyme composition comprising β-glucosidase and one or more amylases under conditions sufficient to hydrolyze cellulose and starch in the milled grain to glucose;
- and incubating the glucose with at least one fermenting microorganism under conditions in which ethanol is produced.
Dependencies are inferred from the claim text.