Methods for delaying crispr action and improving gene drive effectiveness

Abstract

The present disclosure provides the ability of low-cutting gRNAs to circumvent the maternal effect by delaying their action in time. Low-cutting gRNAs lead to a compounding dynamic drive efficiency, which results in drive efficiencies comparable to that of high-cutting gRNAs. Additionally, a gene drive system with a low-cutting gRNA outperforms a more efficient gRNA in laboratory caged populations.

Dependencies are inferred from the claim text.