High-throughput selection and fabrication of biomaterial-encapsulated cell mass and uses thereof

A method for selecting cell masses from a source and fabricating a biomaterial-encapsulated cell mass model (BECM) based on the selected cell masses, the method comprising:

• providing one or more cell masses in an intact or partially digested state;
• subjecting the intact or partially digested cell masses to a cell imaging device;
• providing a formulation of a biomaterial to encapsulate the cell masses in-situ;
• acquiring images of the cell masses to target cell masses to be encapsulated into the biomaterial;
• determining location, dimension, cell number, cell phenotype, and optical signal intensity of the target cell masses with respect to non-targeted cell masses in the images;
• and
• mixing the formulation of the biomaterial with the target cell masses in the corresponding compartment followed by inducing a formation of a network, gelation system or scaffold for encapsulating and accommodating the target cell masses.