Method for extracting epidermal cells

A method for extracting epidermal cells, comprising:

• soaking skin tissues in a digestive juice, and obtaining an epidermal cell suspension through alternate use of a high-temperature digestion condition and a low-temperature digestion condition, the method specifically comprising the following steps:
• 1) taking out the skin tissues in a sterile environment, putting the skin tissues into a culture dish, and washing the skin tissues with normal saline;
• 2) soaking epidermal tissues in the digestive juice to perform digestion, wherein a digestion environment in the digestive juice involves alternation of the high-temperature digestion condition and the low-temperature digestion condition or the low-temperature digestion condition and the high-temperature digestion condition;
• 3) after digestion, separating epidermis from dermis;
• and
• 4) resuspending the epidermal tissues with normal saline to obtain the epidermal cell suspension;
• wherein the method is capable of obtaining the epidermal cell suspension within 4 h, and in the cell suspension, a cell extraction amount is greater than 1×107 cells/10 cm2, and the cell survival rate is higher than 85%.