Pipetting Device, Pipette Tip Coupler, and Pipette Tip: Devices and Methods

A pipette tip comprising:

• a circumscribing sidewall with an open interior passage including an interior surface extending along a central longitudinal axis of the pipette tip between an open proximal end and an open distal end of the pipette tip;
• a first section formed in the interior surface of the circumscribing sidewall distal to the open proximal end, the first section having a first length and a first diameter wherein the first diameter is at a widest portion of the first section;
• a second section formed in the interior surface of the circumscribing sidewall distal to the first section, the second section having a second diameter and the second section having a second length;
• wherein the second diameter is at a widest portion of the second section;
• and
• wherein the second diameter is smaller than the first diameter;
• a first working surface formed in the circumscribing sidewall;
• a stop shoulder surface formed in the interior surface of the circumscribing sidewall at an interface of the first section and the second section;
• a third section formed in the interior surface of the circumscribing sidewall distal to the second section, the third section having a third length;
• a sealing seat formed in the interior surface of the circumscribing sidewall and interposed between the second section and the third section, the sealing seat comprising:
• an upper annular sealing seat surface edge at an interface with the second section;
• a lower annular sealing seat surface edge distal to the upper annular sealing seat surface edge;
• a sealing seat surface interposed between the upper annular sealing seat surface edge and the lower annular sealing seat surface edge;
• and
• wherein the upper annular sealing seat surface edge is greater in diameter than a narrowest portion of the sealing seat surface;
• at least one frustoconical section formed in the interior surface of the circumscribing sidewall and between the third section and the open distal end;
• wherein at least a portion of the first working surface is configured to receive a first radial force and a first axial force from a coupling device inserted into the open interior passage of the pipette tip to secure the pipette tip against the coupling device;
• wherein the first radial force is directed outward and perpendicular from the central longitudinal axis;
• wherein the first axial force is directed upward and parallel to the central longitudinal axis;
• wherein the sealing seat is configured for receiving a second radial force and a second axial force when a coupling device is inserted into the pipette tip to seal the coupling device against the sealing seat of the pipette tip;
• wherein the second radial force is directed outward and perpendicular from the central longitudinal axis;
• and
• wherein the second axial force is directed downward and parallel to the central longitudinal axis.