Methods of Treating Conditions Associated with Leaky Gut Barrier
AssigneeThe Regents of the University of California
InventorsPradipta Ghosh, Soumita Das
Life of this patent
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Abstract
Methods to interrogate and modulate gut barrier integrity are provided. Methods for treating leaky gut barrier are also provided. Methods for early detection of diseases associated with inflammatory disorders. Methods to rapidly assess the effects of drugs, chemicals, nutritional supplements, vitamins, and probiotics on the integrity of the gut barrier are also provided.
Claims17
A method of identifying a compound with an ability to enhance or disrupt the gut barrier comprising:
- combining a candidate compound with an enteroid-derived monolayer derived from a human suffering from inflammatory bowel disease;
- measuring or observing a signal associated with an AMPK→GIV stress-polarity pathway;
- and
- determining that the candidate compound activated the AMPK→GIV stress-polarity pathway.
A method of screening a compound for an ability to enhance or disrupt the expression of MCP-1 in gut epithelium comprising:
- combining a candidate compound with an enteroid-derived monolayer derived from a human suffering from inflammatory bowel disease;
- measuring or observing a signal associated with an ELMO1→MCP-1 signaling axis;
- and
- determining whether the candidate compound activated the ELMO1→MCP-1 signaling axis.
A method of identifying a compound with an ability to enhance or disrupt the gut barrier comprising:
- combining a candidate compound with an enteroid-derived monolayer derived from a human suffering from inflammatory bowel disease;
- measuring the function and/or structure of tight junctions in the monolayer;
- and
- determining if the candidate compound stabilized the tight junctions.
Dependencies are inferred from the claim text.