Drug-loaded liposomes and uses thereof in treating biofilm

A method for treating biofilm in a subject in need thereof, the method comprising administering to the subject a liposome comprising:

• (a) at least one bilayer-forming lipid;
• (b) a polymeric compound having the general formula I:
• wherein:
• m is zero or a positive integer;
• n is an integer which is at least 1, wherein when X does not comprise a phosphate group, n is at least 2;
• X is a lipid moiety;
• Y is a backbone unit which forms a polymeric backbone;
• L is absent or is a linking moiety;
• and
• Z has the general formula II:
• wherein:
• A is a substituted or unsubstituted hydrocarbon;
• B is an oxygen atom or is absent;
• and
• R1-R3 are each independently selected from the group consisting of hydrogen, alkyl, cycloalkyl, heteroalicyclic, aryl and heteroaryl;
• c) a positively-charged lipidic agent, incorporated within a lipid bilayer and/or on a surface of the liposome;
• and
• (d) a therapeutically active agent, bound to a surface of the liposome and/or within a lipid bilayer and/or core of the liposome.