Electroosmotic perfusion with external microdialysis probe
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsStephen G. Weber, Jun Chen, Michael Trey Rerick
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Abstract
A probe can be situated within a tissue to perfuse one or more materials, collect material in response, and pass the collected material to a microdialysis probe situated external to the tissue based on fluid flow by electroosmosis. Perfusion and collection can be via orifices that are offset laterally and along a tissue depth. Variation of perfusion by varying materials perfused or rate of perfusion can be used to determine perfusion rates or other time-dependencies., concentrations of chemical species and rates of reactions of chemical species in the tissue.
Claims33
An electroosmotic (EO) probe, comprising:
- a probe body that a defines a first probe cavity and a second probe cavity, the first probe cavity defining a first orifice adapted to dispense a perfusate and the second probe cavity defining a second orifice situated to receive a collection fluid responsive to the dispensation of the perfusate from the first orifice;
- and
- a first tapered probe tip terminating the first probe cavity and a second tapered probe tip terminating the second probe cavity.
A method, comprising:
- inserting a first probe and a second probe into a tissue, wherein the first probe is fixed with respect to the second probe;
- electro-osmotically providing a perfusate to the tissue from the first probe;
- and
- collecting a fluid from the tissue in response to the perfusate with the second probe.
An electroosmotic (EO) probe, comprising:
- a probe body that defines probe cavity and a tapered probe tip terminating the probe body, wherein the probe body defines an orifice adapted to dispense a perfusate;
- and
- a microdialysis (MD) probe situated to receive a collection fluid responsive to dispensation of the perfusate from the orifice.
Dependencies are inferred from the claim text.