Multivalent viral particles
AssigneeCornell University
InventorHector AGUILAR-CARRENO
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Abstract
The present disclosure is directed to Compositions comprising a population of multivalent viral particles are described herein. Also described herein are immunogenic compositions comprising a population of multivalent viral particles. Additionally, methods of making a multivalent immunogenic composition comprising a population of multivalent viral particles are described. Lastly, methods of using an immunogenic composition to elicit an immune response in a subject are described herein.
Claims63
A composition comprising a population of particles that are replication-incompetent virions or virus-like particles, wherein the particles each comprise
- (i) a matrix glycoprotein base, wherein the matrix glycoprotein is from a first virus species, and
- (ii) surface glycoproteins from at least two virus species.
A method of making a multivalent immunogenic composition, the method comprising:
- providing a host cell comprising (i) a modified genomic DNA of a first virus species comprising a nucleic acid encoding a matrix glycoprotein, wherein the modification creates a replication incompetent virus, comprises a deletion of a gene encoding a surface glycoprotein;
- and (ii) nucleic acids encoding surface glycoproteins from at least 2 virus species;
- infecting the host cell with a helper virus of the first virus species to produce a population of particles comprising replication incompetent virions comprising the matrix protein of the first virus species and the surface glycoproteins from at least 2 virus species;
- and
- isolating the population of particles.
A method of making a multivalent immunogenic composition, the method comprising:
- transfecting a host cell with a nucleic acid encoding a matrix protein of one virus species and nucleic acids encoding surface glycoproteins of at least two virus species;
- expressing viral proteins in the host cell to allow formation of particles comprising surface glycoproteins of at least two virus species;
- purifying the particles;
- formulating the multivalent immunogenic composition comprising the purified particles.
Dependencies are inferred from the claim text.