Inclusion body mediated method for double-stranded rna producing
AssigneeXiaomo Biotech Limited
InventorsLINFENG HUANG, PANPAN HUANG
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Abstract
An inclusion body mediated method for double-stranded RNA producing. The method includes: transforming the vector to bacteria; culturing and inducing the bacteria expressed an inclusion body; and extracting the inclusion body by the sonication or high-pressure lysis of the collected bacteria, wherein the vector at least includes a nucleic acid sequence encoding a siRNA-binding polypeptide that corporate p19 protein with protein 1.
Claims18
A vector comprising:
- a first nucleic acid sequence encoding a siRNA-binding polypeptide comprising p19 protein and protein 1;
- and
- a second nucleic acid sequence substantially complementary to a target RNA.
An insoluble complex, generated by the interaction of a fusion protein of p19 and a double stranded RNAs of target sequence.
An inclusion body for use in the purification of RNA product, wherein the inclusion body is formed by protein 1 fused with RNA-binding protein.
Dependencies are inferred from the claim text.