Loading patent…
AssigneesSESVanderHave NV, DLF Seeds
InventorsHilde Maurice Anne VAN HOUTTE, Shivegowda Shikaranahalli THAMMANNAGOWDA, Murielle Anne Francoise LOMMEL, Glenda WILLEMS, Per Erik SNELL, Ingo Peter LENK, Elisabeth Rosine Armand VEECKMAN
Estimating the term…
The present disclosure relates to Beta vulgaris plants having resistance to PPO herbicides and methods of producing said plants by targeted genome editing. The disclosure further relates to genetic sequences for use with targeted genome editing technologies and/or genotyping, and herbicide-resistant PPO proteins produced from genetically engineered, non-transgenic Beta vulgaris plants.
A method of producing a Beta vulgaris plant with increased tolerance to an herbicide that inhibits protoporphyrinogen oxidase comprising the steps of:
An isolated polynucleotide comprising an engineered nucleic acid sequence encoding a protein comprising an amino acid substitution at a position corresponding to 126 of SEQ ID NO:
A method for producing a Beta vulgaris plant or plant cell having an engineered PPO2 protein comprising:
A guide RNA suitable for use in a CRISPR based genome editing system, wherein said guide RNA is selected from SEQ ID NOs:
A donor template sequence suitable for use in a CRISPR based genome editing system, wherein said donor template sequence is selected from SEQ ID NOs:
An engineered PPO2 protein comprising an amino acid substitution corresponding to position number 126 in SEQ ID NO:
A non-transgenic, genetically engineered Beta vulgaris plant, or part thereof, comprising a nucleic acid encoding a protoporphyrinogen oxidase 2 (PPO2) amino acid sequence, wherein said PPO2 amino acid sequence has been engineered to comprise a substitution of arginine at a position corresponding to 126 of SEQ ID NO:
Dependencies are inferred from the claim text.