Biocompatible optical slide intended for total internal reflection microscopy and microscopy imaging system including such a slide

A total internal reflection microscopy system comprising:

• an optical slide comprising:
• an optically transparent base substrate;
• and
• a stack of layers of dielectric materials, wherein said stack is arranged directly on the base substrate and formed of a succession of pairs of alternating thin layers of a first dielectric material of high refractive index and of a second dielectric material of low refractive index capable of producing an optical resonance at a predetermined angle of incidence and illumination wavelength of the optical slide in total reflection mode;
• a light source configured to emit a light beam;
• and
• a microscope lens configured to form the light beam towards the optical slide;
• wherein the optical slide and the microscope lens are configured so that the angle of incidence is:
• greater than or equal to a critical angle of total internal reflection, and less than or equal to a limit value defined depending on the numerical aperture of the microscope lens.