Polymer coatings

A method of preparing a polymer coating immobilized to a surface of a substrate, said method comprising:

• contacting a polymer with a surface of a substrate, wherein the surface comprises a plurality of functional groups, thereby forming a layer of polymer coating on the surface, and wherein the polymer coating is covalently bonded to the functional groups on the surface,
• wherein the polymer coating comprises a recurring unit of Formula (I) and a recurring unit of Formula (II):
• wherein:
• each R1 and R1′ is independently selected from hydrogen, halo, alkyl, alkoxy, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, or optionally substituted variants thereof;
• each R2 and R3 is independently selected from hydrogen, alkyl, alkylamino, alkylamido, alkylthiol, aryl, or optionally substituted variants thereof;
• each R4, R4′, R5 and R5′ is independently selected from H, OH, OR6, —C(O)OR6, —C(O)R6, —OC(O)R6, —C(O)NR7R8, or —NR7R8;
• R6 is independently selected from H, OH, alkyl, cycloalkyl, hydroxyalkyl, aryl, heteroaryl, heterocyclyl, or optionally substituted variants thereof;
• and
• each R7 and R8 is independently selected from H or alkyl, or R7 and R8 are joined together with the atom or atoms to which they are attached to form a heterocycle.

A method of preparing an array of beads comprising:

• forming a plurality of functional groups on the surface of one or more beads;
• and
• contacting a polymer coating with the beads to form a polymer coating layer on the surface of the beads, wherein the polymer coating is covalently bonded to the functional groups on the surface of the beads, wherein the polymer coating comprises a recurring unit of Formula (I) and a recurring unit of Formula (II):
• wherein:
• each R1 and R1′ is independently selected from hydrogen, halo, alkyl, alkoxy, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, or optionally substituted variants thereof;
• each R2 and R3 is independently selected from hydrogen, alkyl, alkylamino, alkylamido, alkylthio, aryl, or optionally substituted variants thereof;
• each R4, R4′, R5 and R5′ is independently selected from H, R6, OR6, —C(O)OR6, —C(O)R6, —OC(O)R6, —C(O)NR7R8, or —NR7R8;
• R6 is independently selected from H, OH, alkyl, cycloalkyl, hydroxyalkyl, aryl, heteroaryl, heterocyclyl, or optionally substituted variants thereof;
• each R7 and R8 is independently selected from H or alkyl, or R7 and R8 are joined together with the atom or atoms to which they are attached to form a heterocycle.