Method for increasing throughput of single molecule sequencing by concatenating short dna fragments
AssigneeRoche Sequencing Solutions, Inc.
InventorsJanine Mok, Ulrich Schlecht, Austin So
Life of this patent
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Abstract
The invention comprises a method and compositions for sequencing library preparation, which increases the throughput of single-molecule sequencing (SMS) platforms by generating long concatenated templates from pools of short DNA molecules.
Claims6
1-15. (canceled)
A method of making a library of concatenated target nucleic acid molecules from a sample, wherein the method comprises the following steps:
- (a) attaching a first adaptor to each end of a double-stranded target molecule, wherein the first adaptor has at least one-double-stranded region;
- (b) contacting the sample with an exonuclease to generate partially single-stranded adaptor regions at the ends of the target molecule;
- (c) joining at least two target molecules by hybridizing the partially single-stranded adaptor regions on each strand of the target molecules to form the double-stranded adaptor regions and covalently linking the strands of the target molecules, thereby generating concatenated target nucleic acid molecules;
- and
- (d) attaching a second adaptor to the concatenated molecules, wherein the adaptor comprises one or more of:
- barcodes, universal amplification priming sites, and sequencing priming sites, thereby generating a library of concatenated target nucleic acid molecules.
The method of claim 1, wherein the first adaptor is attached by amplifying the target nucleic acid molecules with primers incorporating the adaptor sequences, or the first adaptor is attached by ligation to the ends of the target nucleic acid molecules.
The method of claim 1, wherein the first adaptor comprises a mixture of adaptors capable of ligation on both ends and adaptors capable of ligation on only one end.
The method of claim 1, wherein the first adaptor comprises an exonuclease-resistant region at least about 15 bases from the 5′-end.
A library of concatenated target nucleic acid molecules created using the method comprising:
- (a) attaching a first adaptor having at least one double-stranded region to each end of a double-stranded target molecule;
- (b) contacting the adaptor-containing double-stranded target molecules with an exonuclease to generate partially single-stranded adaptor regions at the ends of the target molecule;
- (c) joining at least two target molecules by hybridizing the partially single-stranded adaptor regions on each strand of the target molecules to form the double-stranded adaptor regions and covalently linking the strands of the target molecules, thereby generating concatenated target nucleic acid molecules;
- and
- (d) attaching a second adaptor to the concatenated molecules, the adaptor comprising one or more barcodes, universal amplification priming sites and sequencing priming sites thereby generating a library of concatenated target nucleic acid molecules.
Dependencies are inferred from the claim text.