Bacteriophages with improved antimicrobial activity
AssigneeArmata Pharmaceuticals, Inc.
InventorsSébastien LEMIRE, Angela B. SORIAGA, Katrina Tram Anh NGUYEN
Life of this patent
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Abstract
Provided herein are bacteriophages engineered to express an exopolysaccharide (EPS) depolymerase for treating bacterial infections. In some embodiments, the EPS depolymerase comprises alginate lyase. Also envisioned within the scope of the invention are compositions comprising one or more of the bacteriophages, methods for treating bacterial infections, and kits comprising the compositions described herein.
Claims91
A bacteriophage engineered to express an exopolysaccharide (EPS) depolymerase.
A bacteriophage composition comprising one or more bacteriophages that express an exopolysaccharide (EPS) depolymerase, wherein the one or more bacteriophages comprise a polynucleotide sequence selected from SEQ ID NO:26;
- SEQ ID NO:27;
- SEQ ID NO:28;
- SEQ ID NO:29;
- SEQ ID NO:30;
- SEQ ID NO:31;
- SEQ ID NO:32;
- SEQ ID NO:33;
- SEQ ID NO:34;
- SEQ ID NO:35;
- SEQ ID NO:37;
- SEQ ID NO:38;
- SEQ ID NO:39;
- SEQ ID NO:
- 40;
- SEQ ID NO:41;
- SEQ ID NO:42;
- SEQ ID NO:43;
- SEQ ID NO:44;
- SEQ ID NO:45;
- SEQ ID NO:46;
- SEQ ID NO:47;
- SEQ ID NO:48;
- SEQ ID NO:49;
- SEQ ID NO:50;
- SEQ ID NO:51;
- SEQ ID NO:
- 52;
- SEQ ID NO:
- 53;
- SEQ ID NO:54;
- SEQ ID NO:55;
- SEQ ID NO:56;
- SEQ ID NO:57;
- SEQ ID NO:58;
- SEQ ID NO:60;
- SEQ ID NO:61;
- SEQ ID NO:
- 62;
- SEQ ID NO:
- 63;
- SEQ ID NO:64;
- SEQ ID NO:65;
- SEQ ID NO:66;
- SEQ ID NO:67;
- SEQ ID NO:68;
- SEQ ID NO:69;
- SEQ ID NO:
- 70;
- SEQ ID NO:71, SEQ ID NO:
- 73;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:26;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:27;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:28;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:29;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:30;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:31;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:32;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:33;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:34;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:35;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:37;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:38;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:39;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:40;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:41;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:42;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:43;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:44;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:45;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:46;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:47;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:48;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:49;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:50;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:51;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:
- 52;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:53;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:
- 54;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:55;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:56;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:57;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:58;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:60;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:61;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:62;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:63;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:64;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:65;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:66;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:67;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:68;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:69;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:70;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:71;
- a polynucleotide sequence with at least 90% identity to SEQ ID NO:73.
The bacteriophage composition of claim 30, wherein the storage medium comprises a cryoprotectant.
A method for making an engineered bacteriophage, comprising providing a bacteriophage and incorporating an exopolysaccharide (EPS) depolymerase into the bacteriophage.
The method of claim 78, wherein the EPS depolymerase is alginate lyase.
A bacteriophage composition comprising one or more bacteriophages engineered to express an exopolysaccharide (EPS) depolymerase, wherein the one or more bacteriophages belong to the Genus Phikmvvirus, Pakpunavirus, Bruynoghevirus, and/or Pbunavirus.
Dependencies are inferred from the claim text.