Gene sequence construct for gene therapy of human immunodeficiency virus infection

Abstract

A gene sequence construct for gene therapy of human immunodeficiency virus (HIV) infection. By sequentially linking, by means of a coding sequence of a linker polypeptide, gene coding sequences of respective single-chain variable fragment (scFv) regions of light chains and heavy chains of monoclonal antibodies having different binding site antigens involved in different steps of infecting human CD4+T cells by an HIV, gene coding sequences of respective scFv regions of light chains and heavy chains of monoclonal antibodies bound to a CD4 receptor site, and a gene coding sequence of a polypeptide for inhibiting the fusion of an HIV and a CD4+T cell membrane, a gene sequence construct is constructed in a promoter and downstream of a secretory signal peptide coding sequence to express a secretory antibody-like protein molecule coded by a single gene. The recombinant single-gene construct can be conveniently introduced into a target tissue cell by means of a viral vector, and a secretory expression antibody or antibody-like protein molecule has multi-antigen antigen tropism, and can effectively and widely block an infection process of an HIV on human CD4+T cells by binding to a plurality of binding sites involved in different steps of infecting human CD4+T cells by an HIV, and effectively avoid losing an HIV infection inhibition capability due to the escape mutation of an HIV, thereby achieving a long-term or even permanent treatment effect on HIV infection by single injection administration.

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