US 2024/0351044 A1 · SYSTEM AND METHOD TO PERFORM QUANTITATIVE POLYMERASE CHAIN REACTION

Abstract

Example methods and systems for performing quantitative polymerase chain reaction (qPCR) are provided. One example system may include a first lighting subsystem and a second lighting subsystem. The first lighting subsystem includes a first light source configured to illuminate a qPCR testing solution including a sample, a fluorescence dye and a nanoparticle in a tube with a first light to increase a temperature of the qPCR testing solution. The second lighting subsystem includes a light detector configured to detect an amount of fluorescence emitted by the fluorescence dye in a first thermal cycle of the qPCR. The first lighting subsystem is configured to turn off the first light source for a period of time in any thermal cycle of the qPCR after the first thermal cycle based on one or more heat transmission parameters which are determined based on the amount of fluorescence.

Claims20

Dependent claims

A system configured to perform quantitative polymerase chain reaction (qPCR), comprising:

a first lighting subsystem including a first light source, wherein the first light source is configured to illuminate a qPCR testing solution including a sample, a fluorescence dye and a nanoparticle in a tube with a first light to increase a temperature of the qPCR testing solution;
and
a second lighting subsystem including a light detector configured to detect an amount of fluorescence emitted by the fluorescence dye in a first thermal cycle of the qPCR,
wherein the first lighting subsystem is configured to turn off the first light source for a period of time in any thermal cycle of the qPCR after the first thermal cycle based on one or more heat transmission parameters which are determined based on the amount of fluorescence.

1 more element

A non-transitory computer readable medium that includes a set of instructions which, in response to execution by a processor of a computing device, cause the computing device to perform quantitative polymerase chain reaction (qPCR), wherein the method comprises:

in a first thermal cycle of performing the qPCR:
determining one or more heat transmission parameters based on an amount of fluorescence emitted by a fluorescence dye included in a qPCR testing solution in a tube;
and
determining a first time duration of turning on a first light source configured to illuminate the qPCR testing solution further including a nanoparticle and a sample with a first light, a second time duration of turning off the first light source, a first power of the first light source and a second power of the first light source based on the one or more heat transmission parameters.

1 more element

Dependencies are inferred from the claim text.

System and method to perform quantitative polymerase chain reaction

Life of this patent

System and method to perform quantitative polymerase chain reaction

Life of this patent

Abstract

Claims20