Compositions and methods for high efficiency genome editing

A method for integrating an exogenous sequence into a chromosomal sequence of a eukaryotic cell, the method comprising:

• a. introducing into the eukaryotic cell:
• (i) at least one RNA-guided endonuclease comprising at least one nuclear localization signal or nucleic acid encoding at least one RNA-guided endonuclease comprising at least one nuclear localization signal,
• (ii) at least one guide RNA or a DNA encoding at least one guide RNA, and
• (iii) at least one donor polynucleotide comprising the exogenous sequence;
• b. generating a double-stranded break a target site in the chromosomal sequence, wherein at least one guide RNA guides the at least one RNA-guided endonuclease to the target site;
• and
• c. repairing the double strand break using a DNA repair process,
• thereby integrating the exogenous sequence into the chromosomal sequence of the eukaryotic cell,
• wherein the efficiency of integrating the exogenous sequence is about 20%, 25%, 30%, 35%, 40%, or 45% higher compared to a reference sample.