Method, an apparatus, an assembly and a system suitable for determining a characteristic property of a molecular interaction

A method for determining a characteristic property of a molecular interaction, the method comprising:

• providing a liquid sample comprising a particle capable of being in a state of equilibrium and in a state of non-equilibrium in said liquid sample, the particle comprises a marker in at least one of its state of equilibrium and state of non-equilibrium;
• bringing the particle in a state of non-equilibrium by subjecting the sample to a condition jump;
• reading out said marker as a function of time comprising at least two readings of intensity of more than one wavelengths from the point of time where the particles are subjected to the condition jump wherein the more than one wavelength comprises a change of wavelength;
• and
• determining said characteristic property of said molecular interaction,
• wherein said condition jump comprises subjecting the sample to a jump in temperature from at least one first temperature to a second condition at a second temperature.