Engineered heparin bioactive matrix for clinical application of blood contacting surface and method of manufacturing the same

A method of manufacturing a medical device having an engineered heparin bioactive matrix on a blood-contacting metal surface, comprising:

• a) activating a blood-contacting metal surface of at least one component of the medical device to form an activated surface via i) propene plasma treatment or ii) contacting the surface with an organic solution comprising a silane functional compound having at least one ethylenically unsaturated functional group, wherein the medical device comprises a pipeline flow diverter or a stent and wherein the organic solution is introduced into and follows a blood flow path through the medical device;
• b) grafting a polymeric hydrogel to the activated surface;
• c) optionally hydrolyzing the polymeric hydrogel to form carboxyl functional groups on the polymeric hydrogel;
• d) bonding a positively charged spacer molecule to the polymeric hydrogel by contacting the polymeric hydrogel with a first wet chemistry treatment composition comprising an aqueous solution containing a cationic polymer, wherein the first wet chemistry treatment follows a blood flow path through the medical device;
• and
• e) covalently bonding heparin to the spacer molecule by contacting the spacer molecule with a second wet chemistry treatment composition comprising heparin, wherein the second wet chemistry treatment follows a blood flow path through the medical device.